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Image Search Results
Journal: Pharmaceutical Biology
Article Title: Chaihu Shugan San promotes gastric motility in rats with functional dyspepsia by regulating Drp-1-mediated ICC mitophagy
doi: 10.1080/13880209.2023.2166966
Figure Lengend Snippet: Effects of CHSGS on the fluorescence co-localization expression of LC3 and VDAC1, Drp-1 and Tom20 in gastric antrum tissue of FD rats. (A) Immunofluorescence of LC3 and VDAC1, (B) Colocalization of LC3 and VDAC1 Immunofluorescence. (C) Immunofluorescence of Drp-1 and Tom20. (D) Colocalization of Drp-1 and Tom20 Immunofluorescence. Data were presented as means ± SD , compared with C, ** p < 0.01, compared with M, ## p < 0.01.
Article Snippet:
Techniques: Fluorescence, Expressing, Immunofluorescence
Journal: The Journal of Neuroscience
Article Title: Neuronal Mitochondria Modulation of LPS-Induced Neuroinflammation
doi: 10.1523/JNEUROSCI.2324-19.2020
Figure Lengend Snippet: Mfn2 overexpression alleviates peripheral LPS-induced mitochondrial fragmentation in neurons. A, Representative single plane (top) and 3D z-stack illustrations (middle/bottom) of immunofluorescent staining for VDAC1 (AF488; green) and nuclei (DAPI; blue) in CA1 neurons of TMFN and NTg mice at 2 dpi. Red boxes indicate region enlarged in the bottom panels (n = 4 mice/group). B, Quantification of mitochondrial length in wide-field VDAC1 (AF488) immunofluorescent images of CA1 neurons of TMFN and NTg mice at 2 dpi. Measurements were averaged for individual CA1 neuronal images from each mouse. Data points represent the average mitochondrial length for individual mice (PBS injection: n = 6 mice/group; LPS injection: n = 5 mice/group). Error bars represent mean ± SEM, representative of triplicate experiments. One-way ANOVA followed by Tukey's multiple-comparison test. ***p < 0.001. ns, Nonsignificant. For further details on mitochondrial dynamics see Figure 4-1. For neuronal viability see Figure 4-2.
Article Snippet: Primary antibodies (all diluted 1:3000 in TBST from stock concentrations): mouse monoclonal anti-Drp1 (611112, BD Biosciences), rabbit monoclonal anti-GAPDH (2118, Cell Signaling Technology), mouse monoclonal anti- glial fibrillary acidic protein (GFAP; 14-9892-82, Invitrogen), mouse monoclonal anti-HIF-1α (610958, BD Biosciences), rabbit monoclonal anti-Iba1 (013-27691, Wako Chemical), rabbit polyclonal anti-MFF (12741, Abcam), mouse monoclonal anti-Mfn1 (sc-166644, Santa Cruz Biotechnology), mouse monoclonal anti-Mfn2 (sc-100560, Santa Cruz Biotechnology), rabbit monoclonal anti-Mfn2 (11925, Cell Signaling Technology), mouse monoclonal anti-Opa1 (612606, BD Biosciences), rabbit polyclonal anti-MAP2 (AB5622, MilliporeSigma), rabbit monoclonal anti-Synaptophysin (5461S, Cell Signaling Technology), and
Techniques: Over Expression, Staining, Injection